A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements
نویسندگان
چکیده
منابع مشابه
A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements.
We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents a pool of approximately 15 000 genomic loci, could be used for direct sequencing, selective restriction digestion or pyrosequencing, in o...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 1362-4962
DOI: 10.1093/nar/gnh032